AHSEC| CLASS 12| BIOTECHNOLOGY| QUESTION PAPER - 2022| H.S. 2ND YEAR
2022
BIOTECHNOLOGY
(Theory)
Full Marks: 70
Pass Marks: 21
Time: Three hours
The figures in the margin indicate
full marks for the questions.
1. (a) What does PCR stand for? 1
(b) Define stem
cell. 1
(c) What are DNA
ligases? 1
(d) What do you
mean by ‘vector’? 1
(e) What is ‘Rh’
factor? 1
(f) What is
bioremediation? 1
(g) What is
Palindromic sequence? 1
(h) Define the
term ‘Totipotency’. 1
2. What is Bioenergy? 2
3. What is cDNA? Name the enzyme responsible for production
of cDNA. 2
4. Briefly write about the rDNA technology. 2
5. Write down the role of media in cell culture process. 2
6. Define the terms ‘Lagging’ and ‘Leading’ strands 2
7. What is Proteomics? 2
8. What are Antibodies? 2
9. Define Gene Therapy. 2
10. What is DNA Probe? 2
11.What is micropropagation? 2
12. Briefly describe the steps involved in the production of
monoclonal antibodies. 3
13. What are restriction enzymes? 3
14. Explain the structure of any one cloning vector. 3
15. Who developed the PCR process? Explain the main steps
involved in the PCR technique. 3
16. What are the transgenic plants? Explain briefly. 3
17. Explain the structure of tRNA. 3
18. What is RELP? Mention the basic steps. 3
19. Write few applications of microbial culture. 3
20. What is electrophoresis? Explain. 3
21. Explain the cDNA library construction process. 5
Or
What is ELISA?
Write down the steps of ELISA testing. 2+3=5
22. Explain the role of microbes as bio-fertilizers. 5
Or
What is
Bioinformatics? Write about the uses of bioinformatics tools. 5
23. Write short notes on: (any two) 2 ½ x2=5
(a) Southern
blotting
(b) IPR
(c) DNA Helicase
(d) Transgenic
animal.
***
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